Fig. 2: CRSP8 promoted the growth of thyroid cancer cells partially by activating PI3K/AKT signaling pathway.
From: CRSP8 promotes thyroid cancer progression by antagonizing IKKα-induced cell differentiation
A–H Thyroid cancer cells were transfected with CRSP8 specific siRNAs or its overexpression plasmids, 48 h after transfection. A, B CRSP8 expression was analyzed by western blot; C, D cell viability was tested by MTT assay; E, F colony formation assay was performed and colony number was counted; G, H the protein levels of CRSP8, p110α, p-p85, p85, p-AKT, AKT were detected by western blot. I, J THJ-21T and BCPAP cells were pretreated with a PI3K inhibitor LY294002 (50 μM) for 4 h, and then transfected with CRSP8 specific siRNAs or overexpression plasmids. After 48 h, cell viability was determined by MTT analysis. The data represent the mean ± SD of three independent experiments, and the level of significance was indicated by ***P < 0.001, **P < 0.01, *P < 0.05, ns nonsignificant (p > 0.05). LY: LY294002.
