Fig. 4: CRSP8 regulated the differentiation of thyroid cancer cells and the negative regulatory effect of CRSP8 on IKKα in thyroid cancer cells and tissues.
From: CRSP8 promotes thyroid cancer progression by antagonizing IKKα-induced cell differentiation
A Western blot analysis of CRSP8, NIS, and Tg expression in thyroid cancer cells transfected with CRSP8 specific siRNAs or its overexpression plasmids. B Western blot analysis of IKKα, NIS, and Tg expression in thyroid cancer cells treated with IKKα specific siRNAs or its overexpression plasmids. C Western blot analysis of the expression of EMT markers (β-catenin, Vimentin, and Slug) in thyroid cancer cells following IKKα knockdown or overexpression. D RT-PCR analysis and E western blot analysis of CRSP8, IKKα expression in thyroid cancer cells following CRSP8 knockdown or overexpression. F The correlation between CRSP8 and IKKα expression was detected using cBioPortal database. G The representative images for CRSP8 and IKKα expression by IHC analysis in human thyroid cancer tissue microarrays. Scale bars, 100 μm. H Percentages of specimens with either low or high CRSP8 expression relative to IKKα level.
