Fig. 5: ESCRT-III modulates cell death and the immunologic outcome of ferroptotic cells.
From: Ferroptotic pores induce Ca2+ fluxes and ESCRT-III activation to modulate cell death kinetics
A Immunoblot showing the knock down of CHMP4B in NIH-3T3 cells. B Effect of knocking down CHMP4B on the kinetics of ferroptosis. C Effect of CHMP4B depletion on the cytokine profile of ferroptotic cells. NIH-3T3 cells were treated with RSL3 (2 µM) for 24 h. Supernatants were filtered, and the cytokine composition was analyzed by the proteome profiler mouse XL cytokine Array kit. D Summary of the cytokines that are regulated in C. Cytokines that are differently regulated upon CHMP4B depletion are highlighted in green. E CD14 expression in the plasma membrane of raw 264.7 macrophages after stimulation with the supernatants obtained from NIH-3T3 cells treated with RSL3, as indicated in C. Each bar represents the mean and standard deviation for three independent experiments. The statistical comparison was carried out by the Student’s t-test. * indicates independent groups with significant differences among them (p < 0.05).
