Fig. 3: TfR2 deletion is protective in 52-week-old MPTP-treated mice. | Cell Death & Differentiation

Fig. 3: TfR2 deletion is protective in 52-week-old MPTP-treated mice.

From: Gender biased neuroprotective effect of Transferrin Receptor 2 deletion in multiple models of Parkinson’s disease

Fig. 3

A Striatal TH immunoreactivity in 52-week-old mice. TfR2 deletion provides moderate, yet significant protection from MPTP treatment. B Unbiased stereological counts of TH immunopositive neurons in the SNpc confirm that TfR2 deletion is protective in females, but not in males. C. Perls’ staining for Fe3+ reveals significant decrease in iron accumulation in the substantia nigra in after MPTP treatment in animals with TfR2 targeted deletion. In 52-week-old mice, TfR2 deletion also reduces accumulation of iron deposits in untreated females. D Double histological staining to reveal Fe3+ in TH immunopositive neurons in the SNpc, in 12- and 52-week-old mice. Iron deposits partially co-localize with dopaminergic neurons (arrows). Iron levels are remarkably more evident in 52-week than in 12-week-old-treated animals. All data are expressed as percentage of the TfR2fl/flTH:Cre−/− vehicle-treated control group. Two-tailed Student’s t test; *p < 0.05, **p < 0.005, ***p < 0.001. Calibration bars: 1 mm in (A), 2 mm in (C) and 50 μm in (D).

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