Fig. 3: Cullin 2 interacts with p14/ARF and mediates its degradation. | Cell Death & Differentiation

Fig. 3: Cullin 2 interacts with p14/ARF and mediates its degradation.

From: Tumor-associated antigen Prame targets tumor suppressor p14/ARF for degradation as the  receptor protein of CRL2Prame complex

Fig. 3

A Downregulation of Cullin 2 induces the accumulation of p14/ARF. HepG2 cells were transfected with Ctrl or Cullins siRNA for 96 h and harvested for immunoblotting using antibodies against p14/ARF and different Cullins with Actin as a loading control. B p14/ARF interacts with Cullin 2 at endogenous level. HepG2 cells were pretreated with MG-132 for 2 h. Cells were harvested and subjected to immunoprecipitation with anti-p14/ARF Ab and immunoblotting with anti-Cullin Abs. C Downregulation of Cullin2 delays the degradation and extends the half-life of p14/ARF. HepG2 and Huh7 cells were transfected with Ctrl or Cullin2 siRNA for 96 h and then treated with 50 μg/mL CHX at indicated time before subjected to immunoblotting analysis using antibody against p14/ARF with Actin as a loading control. The expression of p14/ARF was quantified by densitometric analysis using ImageJ software. All data were representative of three independent experiments. Data represented means, and error bars were standard deviation.

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