Fig. 6: Translocation of XRN1-DCPs into vRC is dependent on RNA length. | Cell Death & Differentiation

Fig. 6: Translocation of XRN1-DCPs into vRC is dependent on RNA length.

From: Spatio-temporal characterization of the antiviral activity of the XRN1-DCP1/2 aggregation against cytoplasmic RNA viruses to prevent cell death

Fig. 6

a (i) In vitro transcribed RNA with indicated length was synthesized and (ii) analyzed on 2% agarose gel with EtBr and PFA. b Non-C.I.A.P-treated RNA species from a were transfected into HeLa cells for 12 h. Immunostaining was performed with indicated antibodies. Nuclei were stained with DAPI. c Percentage of cells with XRN1-DCP1a aggregates from b for indicated experimental conditions (1.0, 2.5, or 5.0 μg of RNA) was quantified. d, e C.I.A.P-treated RNA species from a were transfected into HeLa cells for 12 h, followed by immunostaining and quantification. Treatments with Poly(I:C) and ss-800 nt RNA were used as positive controls, while treatments with total cellular RNA and 100 U/mL of recombinant IFN-β were used as negative controls. XRN1-DCPs aggregates were indicated by white arrows. n.d., not detected. P-value was calculated by Student’s unpaired t-test by comparing to control of respective concentrations.

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