Fig. 7: Mono-, tri-phosphate, and multibranch loop structures of vRNAs are dispensable for the formation of XRN1 aggregates. | Cell Death & Differentiation

Fig. 7: Mono-, tri-phosphate, and multibranch loop structures of vRNAs are dispensable for the formation of XRN1 aggregates.

From: Spatio-temporal characterization of the antiviral activity of the XRN1-DCP1/2 aggregation against cytoplasmic RNA viruses to prevent cell death

Fig. 7

a Total RNA extracted from mock- or NDV-infected HeLa cells was treated with or without XRN1 recombinant enzyme. cDNA was then synthesized. NDV N mRNA was measured by PCR and analyzed on 2% agarose gel. b In vitro synthesized RNA treated under indicated conditions for 12 h was analyzed on agarose gel containing PFA. c (i) In vitro synthesized RNA as indicated was transfected into HeLa cells for 12 h. Immunostaining was carried out with XRN1 antibody. (ii) Percentage of cells with XRN1 foci for indicated experimental conditions was quantified. d HeLa cells were transfected with (i) intact, or (ii) denatured HCV 5′-UTR RNA, followed by immunostaining with XRN1 antibody. (iii) Percentage of cells with XRN1 foci was quantified. All the white scale bars correspond to 10 μm. n.d., not detected. P-value was calculated by Student’s unpaired t-test by comparing to 5′-OH control.

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