Fig. 4: Mdivi-1 treatment reduces Drp1-KLC1 binding and mitochondrial movements.

a Succinate dehydrogenase (SDH) staining of TA muscle sections from Drp/MC mice treated or not with Mdivi-1 (12.5 mg/kg). Percentage of rubbed-out fibres were quantified and shown as percentage of total fibres (n = 3 mice per condition) (scale bar = 50 µm). b Representative immunoblotting of Drp1 and GAPDH as loading control in TA lysates from WT and Drp/MC mice treated or not with Mdivi-1. (n ≥ 3 mice per condition). c Stills from movies showing the area occupied by mitochondria from Drp/MC myotubes treated or not with Mdivi-1 at  10 s (magenta) and after 600 s (blue) (scale bar = 5 µm). Displacement Index was calculated (n ≥ 3 independent experiments). * vs untreated Drp/MC myotubes (**P < 0.01). d Instantaneous velocities of mitochondria in selected ROI were determined in Drp/MC myotubes treated or not with Mdivi-1 using Mytoe software (n ≥ 3 independent experiments). e Representative PLA Drp1:KLC1 (red puncta) on TA muscle sections from Drp/MC mice treated or not with Mdivi-1. DAPI nuclear counterstaining (blue) is provided (scale bar = 25 µm). PLA puncta were quantified and shown as a percentage relative to untreated Drp/MC mice (n = 3 mice per condition). f Co-immunoprecipitation of Drp1 and KLC1 in gastrocnemius lysates from Drp/MC mice treated or not with Mdivi-1. Control immunoglobulins (cIgG) were used as IP negative control. g Representative PLA KIF5B:α-Tubulin (red puncta) on TA muscle sections from Drp/MC mice treated or not with Mdivi-1. DAPI nuclear counterstaining (blue) is provided (scale bar = 25 µm). PLA puncta were quantified and shown as a percentage relative to untreated Drp/MC mice (n = 3 mice per condition). Values are expressed as mean ± SEM. * vs untreated Drp/MC mice (*P < 0.05, **P < 0.01, ***P < 0.001).