Fig. 8: Inactivation of CDC20 drives epidermal hyperplasia into cell death.
From: Squamous differentiation requires G2/mitosis slippage to avoid apoptosis
a H/E stain of epidermis of the tail of control (CDC20+/+; Cre) or KO mice (CDC20 KO; Cre) after 7 or 10 days upon topical treatment with vehicle only or TAM and TPA, as indicated. Note abnormal metaphases (black arrows) and apoptotic bodies (red arrows) in the KO mice. Broken line for the basement membrane. Scale bars: 50 μm. b Percent of 4C (G2/M + tetraploids) or >4C (polyploid) or c apoptotic sub-G1 cells directly isolated from epithelia in a, as indicated. **P < 0.01 *P < 0.05. Data are mean ± SD of three animals per group (N = 3) and two independent experiments. See also Supplementary Fig. 8. d Model for the control of squamous differentiation by the mitotic checkpoint molecules studied. Depletion Cdk1 or Plk1 results in mitotic slippage and endoreplication and differentiation. However, inhibition of mitotic slippage by complete inactivation of CDC20 impairs squamous differentiation and results in apoptosis. Met metaphase; Cyt cytokinesis.
