Fig. 2: Exportin-1-mediated nuclear export of HP1γ contributes to p53 degradation.
From: HPV-mediated nuclear export of HP1γ drives cervical tumorigenesis by downregulation of p53
a Immunoblot analysis of HP1γ immunoprecipitates (IP) and whole-cell lysates (WCL) from HeLa cells treated with or without LMB. b Immunoblot analysis of cytoplasmic and nuclear extracts from SiHa cells treated with or without LMB. c Immunoblot analysis of SiHa cells treated with or without LMB. d SiHa cells were treated with or without LMB, followed by ChIP-qPCR analysis with a p53 antibody in the promoter regions of p53 target genes. e Immunoblot analysis and the mRNA levels of p53 target genes in SiHa cells expressing siRNA of p53 in the presence or absence of LMB. f Representation of HP1γ protein and partial CD sequence containing nuclear export signal (NES) sequences (L150 and L152) which are substituted with alanine in the mutant vector, HP1γ AA. g Immunoblot analysis of HP1γ immunoprecipitates (IP) and whole-cell lysates (WCL) from SiHa cells expressing GFP-HP1γ WT or AA mutant. h Immunofluorescence image of GFP-HP1γ WT or AA mutant expressed in HeLa (left) and SiHa (right) cells. i Immunoblot analysis of cytoplasmic and nuclear extracts from SiHa cells expressing GFP-HP1γ WT or AA mutant. j Immunoblot analysis of SiHa cells expressing GFP-HP1γ WT or AA mutant. k The mRNA levels of p53 target genes in SiHa cells expressing GFP-HP1γ WT or AA mutant. Data are presented as the mean ± SEM (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001.
