Fig. 5: HP1γ negatively regulates p53 ubiquitination by suppressing UBE2L3 expression.
From: HPV-mediated nuclear export of HP1γ drives cervical tumorigenesis by downregulation of p53
a Immunoblot analysis of HeLa cells co-expressing siRNA of HP1 isoforms. b The mRNA levels of UBE2L3 in HeLa cells co-expressing siRNA of HP1 isoforms. c The mRNA levels of TP53 in HeLa cells co-expressing siRNA of HP1 isoforms. d Immunoblot analysis of p53 immunoprecipitates (IP) and whole-cell lysates (WCL) from HeLa cells co-expressing siRNA of HP1 isoforms after MG-132 treatment. e The mRNA levels of p53 target genes in HeLa cells co-expressing siRNA of HP1 isoforms. f HeLa cells were transfected with siRNA of HP1β, followed by ChIP-qPCR analysis for HP1α and HP1γ antibodies in the promoter region of the UBE2L3 gene. g SiHa cells were treated with or without LMB, followed by ChIP-qPCR analysis with an HP1γ antibody in the promoter region of the UBE2L3 gene. h The mRNA levels of UBE2L3 in SiHa cells treated with or without LMB. i SiHa cells were transfected with GFP-HP1γ WT or AA mutant vectors, followed by ChIP-qPCR analysis with a GFP antibody in the promoter region of UBE2L3 gene. j The mRNA levels of UBE2L3 in SiHa cells expressing GFP-HP1γ WT or AA mutant. k Immunoblot analysis of HeLa cells co-expressing siRNA of HP1γ and UBE2L3. l The mRNA levels of UBE2L3 in HeLa cells co-expressing siRNA of HP1γ and UBE2L3. m The mRNA levels of TP53 in HeLa cells co-expressing siRNA of HP1γ and UBE2L3. n The mRNA levels of p53 target genes in HeLa cells co-expressing siRNA of HP1γ and UBE2L3. Data are presented as the mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001.
