Fig. 6: Overexpression of HP1γ promotes p53 signaling by directly suppressing UBE2L3 expression.
From: HPV-mediated nuclear export of HP1γ drives cervical tumorigenesis by downregulation of p53
a Immunoblot analysis of HeLa cells expressing GFP-HP1γ. b The mRNA levels of UBE2L3 in HeLa cells expressing GFP-HP1γ. c Immunoblot analysis of p53 immunoprecipitates (IP) and whole-cell lysates (WCL) from HeLa cells expressing GFP-HP1γ after MG-132 treatment. d The mRNA levels of TP53 in HeLa cells expressing GFP-HP1γ. e ChIP-qPCR analysis with a p53 antibody in the promoter regions of p53 target genes in HeLa cells expressing GFP-HP1γ. f The mRNA levels of p53 target genes in HeLa cells expressing GFP-HP1γ. g Immunoblot analysis of HeLa cells expressing GFP-HP1γ. h The growth rate of HeLa expressing GFP-HP1γ. i Schematic representation of HP1γ protein and partial CD sequence containing V32. j HeLa cells were transfected with GFP-HP1γ WT or mutant (V32A, I165E, or W174A) vectors, followed by ChIP-qPCR analysis with a GFP antibody in the promoter regions of the UBE2L3 gene. k The mRNA levels of UBE2L3 in HeLa cells expressing GFP-HP1γ wild-type (WT) or V32A mutant. l Immunoblot analysis of HeLa cells expressing GFP-HP1γ WT or V32A mutant. m The mRNA levels of p53 target genes in HeLa cells expressing GFP-HP1γ WT or V32A mutant. Data are presented as the mean ± SEM (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001.
