Fig. 6: The combination of ML364 and 17-AAG effectively suppresses HCC1954 xenograft growth. | Cell Death & Differentiation

Fig. 6: The combination of ML364 and 17-AAG effectively suppresses HCC1954 xenograft growth.

From: The deubiquitylase USP2 maintains ErbB2 abundance via counteracting endocytic degradation and represents a therapeutic target in ErbB2-positive breast cancer

Fig. 6

a HCC1954 cells were inoculated into female nude mice to generate xenograft mouse models. Mice were randomized into four groups to receive control vehicle, ML364, 17-AAG, or the combination of ML364 and 17-AAG. The sizes of xenografts were measured every 2 days and calculated tumor volumes were plotted. b Resected xenograft tumor samples from each treatment group. c Tumor weights were measured and plotted. d Tumor tissue sections were analyzed by immunohistochemistry using anti-ErbB2, anti-Ki-67, and anti-cyclin D1 antibodies. Images show representative xenograft sections. Scale bar = 50 μm. e Column charts show quantification data (integral optical density) of relative intensities for ErbB2, Ki-67, and cyclin D1. Error bars represent standard error of the mean (n = 5), with *p < 0.05 and **p < 0.01.

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