Fig. 7: The combined treatment with ML364 and 17-AAG inhibits tumor growth in a syngeneic xenograft mouse model.

a 4T1 cells stably expressing ErbB2 (4T1-ErbB2) were implanted into female Balb/c mice for xenograft formation. Mice were randomly divided into four groups and administered with control vehicle, ML364, 17-AAG, or the indicated combination. The xenograft volumes were measured and plotted. b Xenografts resected from mice receiving different treatment as indicated. c Tumor weights of xenograft samples from each treatment group. d Immunoblotting analysis of tumor tissue samples using indicated antibodies to detect ErbB2 levels. Vinculin blots show equal loading. Column chart shows relative quantification of ErbB2 expression. Error bars represent standard error of the mean (n = 5), with *p < 0.05.