Table 3 Medium components for the culture of organoids derived from the human FRT and placenta.

From: Organoid systems to study the human female reproductive tract and pregnancy

Type

Expansion medium componentsa

Differentiation medium components

Reference

Ovarian

Wnt CM, RSPO1 CM, Noggin, Nicotinamide, EGF, A83-01, Heregulinβ-1, Y-27632, Forskolin, Hydrocortisone, β-Estradiol

–

[69]

Fallopian Tube

Wnt3A CM, RSPO1 CM, Noggin, Nicotinamide, EGF, FGF10, Y-27632, SB431542

β-estradiol, progesterone

[71]

Endometrial

RSPO1, Noggin, Nicotinamide, EGF, FGF10, HGF, A83-01

β-estradiol, progesterone, cAMP, PRL, hPL, HCG

[78]

Wnt3A CM, RSPO1 CM, Noggin, Nicotinamide, EGF, FGF10, A83-01, Y-27632, SB202190, ITS, β-Estradiol

β-estradiol, progesterone

[77]

RSPO1, Noggin, EGF, A83-01, CHIR99021, PGE2

β-estradiol

[79]

Cervical

Endocervix: Wnt3A CM, RSPO1 CM, Noggin, Nicotinamide, EGF, FGF10, SB431542, Y-27632

–

[94]

Ectocervix: Noggin, Nicotinamide, EGF, FGF10, SB431542, Y-27632, Forskolin, Hydrocortisone

  

Trophoblast

RSPO1, Noggin, EGF, HGF, A83-01, CHIR99021, PGE2

Withdrawal of RSPO1 and CHIR99021

[100]

RSPO1, Noggin, EGF, HGF, FGF2, A83-01, CHIR99021, Y-27632, PGE2

Adapted from Okae 2018: 2-mercaptoethanol, BSA, ITS-X supplement, NRG1, A83-01, KOSR; after cell outgrowth (d7-10) NRG1 was omitted

[101]

  1. The medium composition for culturing organoid models of normal FRT tissue and placenta are listed. The growth factors and inhibitors are shown and the basal medium components (e.g., DMEM/F12, N2, B27, N-acetyl cysteine, Hepes, antibiotics) are not listed.
  2. RSPO1 R-spondin 1, EGF epidermal growth factor, FGF fibroblast growth factor, HGF hepatocyte growth factor, ITS insulin transferrin selenium, A83-01 TGFβ receptor inhibitor, SB431542 TGFβ receptor inhibitor, SB202190 p38 MAPK inhibitor, Y-27632 ROCK signaling inhibitor, CHIR99021 GSK-3 inhibitor, PGE2 prostaglandin E2, cAMP 8-Bromoadenosine 3’5’-cyclic monophosphate, PRL Prolactin, hPL human Placental Lactogen, HCG Human Chorionic Gonadotropin, NRG1 Neuregulin 1, KOSR Knockout Serum Replacement, BSA Bovine Serum Albumin, CM conditioned medium.
  3. aExcluding basal medium components.
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