Table 1 Constructed sequences used in this study.

From: CircRNA-vgll3 promotes osteogenic differentiation of adipose-derived mesenchymal stem cells via modulating miRNA-dependent integrin α5 expression

Name

Sequence (5′-3′)

Forward (5-3′)

Annealing temperature (°C)

Product size (base pairs)

CircRNA-vgll3

ACAGCTCAGCTCTTTCAAGCCAGCGGAGTAATTTTCCAACTTCCTTTTGGACCAGCTCTTACCAGCCCCCACCGGCACCTTGTTTGGGGGGAGTTCATCCTGACTTCCAAGTCACTGCACCCCACGGCACCTTTACAACAGCAGACCCCAACTCTTGGCCAGGACATGGTCTGCATCAGACTGGCCCTGCCCCACCCCCTACTGCATCTGAGTCTTGGCACTATCCTCTGGCATCTCAGGTGAGCCCATCCTACAGCCACATGCATGACATGTACATGCGGCATCACCATCCTCATGCTCACATGCACCACCGCCACCACCACCATCACCACCACCCAACTGCTGGCTCTGCCCTGGATCCCGCGTATGGGCACCTGCTGATGCCATCAGTGCGTGCTGCCAGGATTCCTGCTCCCCAGTGTGACATCACAAAGACAGATCTGACTACAGCCACCACTGCTACCTCAGCATGGGCCGGAGCCTTTCATGGGACAGTGGACATCGTGCCAAGTGTGGGCTTCGAAACAG

CircRNA-vgll3-shRNA1

GGCTTCGAAACAGACAGCTCAGCTC

CircRNA-vgll3-shRNA2

GTGGGCTTCGAAACAGACAGCTCAG

CircRNA-vgll3-shRNA3

CGAAACAGACAGCTCAGCTCTTTCA

Si-vgll3

Sense: CCCUACAGCCUGCUAUCAUTT antisense: AUGAUAGCAGGCUGUAGGGTT

miR-326-5p

CAGGCCCTGGACTCAGCTGAGCTTGACTACTTCAGAAGCTGTGGCAAGACACGCAGACCTGGACCAGGGAGGGCTCTGCTCCAGAAGAGTTCTTCCGAGTCTCATCTGTCTGTGGGGCTGGGGGCAGGGCCTTTGTGAAGGCGGGTTATGCTCAGATCGCCTCTGGGCCCTTCCTCCAGTCCCGAGGCAGATTTACCTCGAGGCAAAGCAGCACACCAGCTCCAGGCTCCCTCAGGGCCTCATCTTTTGTGTCCCAGGTCTCAGAGCTCGCCTGAATTTGTAAAAGAAAGATCGTTTTTT

miR-326-5p inhibitor

TTCACAAAGGCCCTGCCCCC

Itga5

CCTGGAACCTCAGCCTGACCAAACAGGCTCACTCATTCCCCAGAGAACGGGAAGCCCCAGGCCTGTACGACCTGGGTTCTGCCCACCAGCTGCACTGACGCTGCCCCTCTCTCCCTAGCCAACCCTCCCCTCTCCTCCAACCCCCCCAACTTATTTAAACTCTGTTGCAAGTGCAATAAACCCCACTCACTGCCCCACTGACC

Itga5 mut

CCTGGAACCTCAGCCTGACCAAACAGGCTCACTCATTCCCCAGAGAACGGGAAGCCCCAGGCCTGTACGACCTGGGTTCTGCCCACCAGCTGCACTGACGGACGGGGTCTCTCCCTAGCCAACCCTCCCCTCTCCTCCAACCCCCCCAACTTATTTAAACTCTGTTGCAAGTGCAATAAACCCCACTCAGACGGGGCTGACC

circRNA-vgll3-wt 1

TTTGGGGGGAGTTCATCCTGACTTCCAAGTCACTGCACCCCACGGCACCTTTACAACAGCAGACCCCAACTCTTGGCCAGGACATGGTCTGCATCAGACTGGCCCTGCCCCACCCCCTACTGCATCTGAGTCTTGGCACTATCCTCTGGCATCTCAGGTGAGCCCATCCTACAGCCACATGCATGACATGTACATGCGGCATCACCATCCTCATGCTCACATGCACCACCGCCACCACCACCATCACCACCACCCAACTGCTGGCTCTGCCCTGGATCCCGCGTATGG

circRNA-vgll3-mut 1

TTTGGGGGGAGTTCATCCTGACTTCCAAGTCACTGCACCCCACGGCACCTTTACAACAGCAGACCCCAACTCTTGGCCAGGACATGGTCTGCATCAGACTCCGGGACGGGGACCCCCTACTGCATCTGAGTCTTGGCACTATCCTCTGGCATCTCAGGTGAGCCCATCCTACAGCCACATGCATGACATGTACATGCGGCATCACCATCCTCATGCTCACATGCACCACCGCCACCACCACCATCACCACCACCCAACTGCTCCGAGACGGGAGGATCCCGCGTATGG

circRNA-vgll3-wt 2

CCACCACCACCATCACCACCACCCAACTGCTGGCTCTGCCCTGGATCCCGCGTATGGGC

circRNA-vgll3-mut 2

CCACCACCACCATCACCACCACCCAACTGCTCCGAGACGGGAGGATCCCGCGTATGGGC

circRNA-vgll3-wt

AGGACATGGTCTGCATCAGACTGGCCCTGCCCCACCAACTGCTGGCTCTGCCCTGGATCCCGCGTATGGGC

circRNA-vgll3-mut

AGGACATGGTCTGCATCAGACTCCGGGACGGGGACCAACTGCTCCGAGACGGGAGGATCCCGCGTATGGG

Itga5-shRNA1

TGGCTATGTCACTGTCCTTAA

Itga5-shRNA2

TGGCATGCGCTCCACTGTATA

Itga5-shRNA3

GGGCACCCAAGGCTAACACTA

Itga5-shRNA4

GCAGATCTCGGAGTCCTATTA

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