Table 2 Primers used for quantitative RT-PCR.
Genes | Forward (5′-3′) | Reverse (5-3′) | Annealing temperature (°C) | Product size (base pairs) |
|---|---|---|---|---|
Col1a1 | GACTGTCCCAACCCCCAAAA | CTTGGGTCCCTCGACTCCTA | 60 | 102 |
Runx2 | ATCATTCAGTGACACCACCA | GTAGGGGCTAAAGGCAAA AG | 60 | 141 |
OSX | GGA AAA GGAGGCACA AAGAA | CAGGGG AGA GGAGTCCAT T | 60 | 106 |
OPN | CCAGCCAAGGACCAACTACA | AGTGTTTGCTGTAATGCGCC | 60 | 132 |
OCN | CAGACA AGTCCCACACAGCA | CCAGCAGAGTGAGCAGAG AG | 60 | 76 |
Itga5 | CAG ATAGCA CCCGAGTTACCA | ACAATCCTACCTGCCCTA ACG | 60 | 113 |
Vgll3 | CCAACACCTTGCATCCCGAA | GGTCTGCTGTTGTAAAGGTGC | 60 | 213 |
AdipoQ | CCACCCAAGGAAACTTGTGC | GACCAAGAACACCTGCGTCT | 60 | 136 |
SOX9 | GTCGGTGAAGAATGGGCAAG | GACCCTGAGATTGCCCGGA | 60 | 161 |
GAPDH | AAGAAACCCTGGACCACCCAGC | TGGTATTCGAGAGAAGGGAGGG | 60 | 178 |
CircRNA-vgll3 | GGAGCCTTTCATGGGACAGT | CTGCTGTTGTAAAGGTGCCGTG | 60 | 196 |
