Fig. 4: KDM4C prevents H3K9me3 accumulation at the TGF-β2 promoter to activate the TGF-β/Smad signaling pathway. | Cell Death & Differentiation

Fig. 4: KDM4C prevents H3K9me3 accumulation at the TGF-β2 promoter to activate the TGF-β/Smad signaling pathway.

From: USP9X-mediated KDM4C deubiquitination promotes lung cancer radioresistance by epigenetically inducing TGF-β2 transcription

Fig. 4

a Volcano plot comparing control and KDM4C knockdown SPC-A1 cells. b Enrichment of differentially expressed genes in signaling pathways is illustrated in an advanced bubble chart. Y-axis represents pathways, and the X-axis represents rich factor. Size and color of the bubble are representation of the amount of differentially expressed genes enriched in pathways and their enrichment significance, respectively. c Heat map produced from RNA sequencing. d, e mRNA levels of indicated genes were measured using quantitative real-time PCR. ***P < 0.001 (n = 3). f–h An H3K9me3 ChIP assay was performed in shKDM4C and shControl cells. Left panel: ChIP primers were designed spanning from −2000 to +200 bp around the transcription start sites of indicated genes. Middle panel: Representative images of gel electrophoresis. Right panel: The H3K9me3 level in the gene promoter region was quantified and normalized to the input. IgG was used as the negative control. *P < 0.05; **P < 0.01; ***P < 0.001 (n = 3).

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