Fig. 5: KDM4C is a key activator of the TGF-β2/Smad/ATM signaling pathway. | Cell Death & Differentiation

Fig. 5: KDM4C is a key activator of the TGF-β2/Smad/ATM signaling pathway.

From: USP9X-mediated KDM4C deubiquitination promotes lung cancer radioresistance by epigenetically inducing TGF-β2 transcription

Fig. 5

a, b Changes in essential components of the TGF-β signaling pathway after genetical or pharmacological inhibition of KDM4C in SPC-A1 cells (n = 3). c, d SPC-A1 cells stably expressing control or KDM4C shRNAs were exposed to irradiation and collected 4 h later. Immunostaining was performed to examine p-ATM and p-Chk2 foci formation. ***P < 0.001 (n = 3). Scale bar, 10 μm. e, f SPC-A1 cells were treated with SD70 (3 μM) for 24 h and 48 h, respectively prior to exposure to irradiation. Cells were harvested after 4 h and subjected to immunostaining using p-ATM and p-Chk2 antibodies. Foci formation was quantified under a fluorescent microscope. ***P < 0.001 (n = 3). Scale bar, 10 μm.

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