Fig. 3: HT-29 and HeLa cells have comparable PKA levels but differ in PKA-dependent phosphorylation.
From: PKA compartmentalization links cAMP signaling and autophagy
A Western blotting of PKA components in total cell lysates of HT-29 and HeLa cells. Both cell lines expressed similar levels of PKA catalytic subunit (PKA Cα) as well as PKA regulatory subunits I and II (PKA RII and PKA RI). GAPDH was used as loading control. Representative data of three independent experiments. B Western blotting of phospho-bands assessed by a phospho-PKA substrate antibody, RRX(S/T)P of total cell lysates (40 µg) of HT-29 and HeLa cells treated with FSK alone (5 µM), FSK (20 µM) combined with IBMX (500 µM), 8CPT-cAMP (5 µM), or the PKA inhibitor H89 (30 µM). C Intensities of phospho-bands, normalized to GAPDH, for HT-29 and HeLa (D). Averages ± SD from at least three independent experiments.
