Fig. 1: Increased FASN expression is associated with an immature AML blast phenotype.

A FASN mRNA levels in AML blasts, CD34⁺ progenitor cells and granulocytes from healthy donors were quantified by qPCR. All the samples were obtained from the Inselspital, Bern, Switzerland. AML patient cells and granulocytes were isolated using Ficoll gradient density centrifugation. Values are the differences in Ct-values between FASN and the housekeeping genes HMBS and ABL1. MNW *p < 0.05, **p < 0.0. B Blood spot data bank analysis of FASN expression in AML blasts compared to granulocytes from healthy donors. MNW *p < 0.05, **p < 0.01. C Western blot analysis of FASN regulation in NB4 and HT93 APL cells upon ATRA treatment at different time points (1, 2 and 3 days). Total protein was extracted and submitted to immunoblotting using anti-FASN antibody. Total protein is shown as loading control. The relative protein expressions were normalized to total protein and quantified using ImageJ software (NIH, Bethesda, MD, USA). Data are represented as a mean (n = 3), Error bars: SD. D Evaluation of FASN transcript levels upon ATRA treatment was done by qPCR. Values were normalized to the HMBS housekeeping gene. Results of at least three independent experiments are shown as n-fold regulation compared with non-treated cells.