Fig. 5: MCL-1 dependence requires BAX/BAK.
From: Breast cancer dependence on MCL-1 is due to its canonical anti-apoptotic function
A Tumoursphere assay with indicated CRISPR/Cas9-modified MDA-MB-231 cell lines, 1 µM S63845 was added at time of plating and spheres were counted at day 7. Points represent independent experiments expressed as % tumoursphere formation relative to untreated BAX/BAK-deficient control. Bars represent mean ± SD of three biological replicates. One-way ANOVA analysis with Tukey’s correction for multiple comparisons. *P < 0.05, ***P < 0.001, n.s. not significant. B Correlation of MCL1 expression with, SNAI2, VIM, CDH3, CD44 and ALDH1A1 across TCGA breast cancers (979 tumours [52]). *Pearson correlation P < 0.05. ER-negative status indicated by black bar in top row. C Comparison of VIM, SNAI2, CDH3 and ALDH1A1 expression in low MCL1 (lower quartile) and high MCL1 (upper quartile) with ER status indicated (red dots for ER-negative, blue dots for ER-positive tumours) TCGA dataset as in B.
