Fig. 2: Defective repair of DNA damage in cells expressing PTEN 398A. | Cell Death & Differentiation

Fig. 2: Defective repair of DNA damage in cells expressing PTEN 398A.

From: The PTEN and ATM axis controls the G1/S cell cycle checkpoint and tumorigenesis in HER2-positive breast cancer

Fig. 2

A Representative images of Pten+/+ and Pten398A/398A MEF cells exposed or not (CTR) to 10 Gy of ionizing radiation, fixed at the indicated times post-IR, and immunostained for γH2AX, and 53BP1. DAPI was used to stain DNA. B Quantification of the number of γH2AX nuclear foci in MEF cells expressing PTEN WT or PTEN 398A. Nuclear foci in at least 100 nuclei were counted. (t test, ****denotes p < 0.0001. C Quantification of the number of 53BP1 nuclear foci in Pten+/+ and Pten398A/398A MEF cells. Mean and standard error of the mean are indicated for each transfection. Nuclear foci in at least 100 nuclei were counted. ****denotes p < 0.0001.

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