Fig. 4: Altered cell cycle progression following DNA damage exposure in cells expressing PTEN 398A. | Cell Death & Differentiation

Fig. 4: Altered cell cycle progression following DNA damage exposure in cells expressing PTEN 398A.

From: The PTEN and ATM axis controls the G1/S cell cycle checkpoint and tumorigenesis in HER2-positive breast cancer

Fig. 4

A–C Cell cycle profiles, assessed by flow cytometry, of MCF10A cells expressing PTEN-WT or PTEN-398A. Cells were either untreated or treated with IR (5 Gy). The captions (and corresponding quantification on the right) correspond to asynchronous cells (asy, A), cells arrested by double thymidine block (0 h, B) and cells synchronized by double thymidine block, released in full media, treated with IR and collected after 6 h (C). The experiment was repeated 3 times; a representative example is shown. D Phospho-H3 and DNA content, assessed by flow cytometry, in MCF10A cells expressing PTEN-WT or PTEN-398A that were synchronized by double thymidine block, released in full media, treated with IR and collected after 6 h. The figure is representative of three different experiments.

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