Fig. 6: Phosphorylation by ATM of PTEN in position 398 mediates its relocalization to the membrane. | Cell Death & Differentiation

Fig. 6: Phosphorylation by ATM of PTEN in position 398 mediates its relocalization to the membrane.

From: The PTEN and ATM axis controls the G1/S cell cycle checkpoint and tumorigenesis in HER2-positive breast cancer

Fig. 6

A Representative images of Pten+/+ and Pten398A/398A MEFs cells exposed or not (CTR) to 10 Gy of ionizing radiation, fixed at the indicated times post-IR, and immunostained for PTEN. B Quantification of membrane-associated PTEN fluorescent signal, relative to the total membrane area of the cell (t test, ****p < 0.0001). C Immunoblot analysis of membrane-associated PTEN in Pten+/+ and Pten398A/398A MEFs cells, exposed or not (CTR) to 10 Gy of ionizing radiation. D Quantification of immunoblot data shown in panel (G). E Model of the effect of phosphorylation of PTEN by ATM on PTEN subcellular localization, PI3K pathway activity, and CDK2/CycE-dependent cell cycle checkpoint regulation, in response to DNA damage.

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