Fig. 1: BCL-2 and BCL-XL are essential for ISC survival during transformation.
From: BCL-XL is crucial for progression through the adenoma-to-carcinoma sequence of colorectal cancer
a Overview of the organoid clonogenic assay. b–d Graphs depict relative outgrowth (number of organoid structures relative to the number prior to treatment and reseeding) of proximal small intestine (SIP)-derived organoid structures quantified 3 days after passaging for the indicated genotype, either without treatment or upon treatment with 1 µM b ABT-199, c A-1155463 and WEHI-539, or d AZD5991, at the time of induction. Each dot represents a replicate (minimal n = 7 per condition, n = 3 independent experiments), error bars indicate s.e.m. ***p < 0.001, ****p < 0.0001, student’s t-test. e, f Graphs depict relative outgrowth of colon-derived organoid structures quantified 3 days after passaging for the indicated genotype, upon treatment with 1 µM e ABT-199 and A-1155463 or f AZD5991, at the time of induction. Each dot represents a replicate (minimal n = 5 per condition, n = 2 independent experiments), error bars indicate s.e.m. *p < 0.05, **p < 0.01, ordinary one-way ANOVA.
