Fig. 6: MiR-17-5p regulates BCL-2 expression in APC-mutant organoids.
From: BCL-XL is crucial for progression through the adenoma-to-carcinoma sequence of colorectal cancer
a BCL-2 mutation and methylation status in CRC tumors of the TCGA COAD dataset, analyzed on cBioPortal. b mRNA expression of BCL-2 in decitabine treated APCKO organoids (n = 2 independent experiments). Error bars indicate s.e.m. c Single cell karyotype-seq showing the ploidy in individual cells of TA1 and TA5. Graphs show individual cells (horizontal lines) and colors indicate copy number state for a given chromosome (columns). d, e qRT-PCR analysis of d miR-17-5p and e miR-18a-3p expression in human normal, APCKO and TA cultures, normalized to U6 snRNA expression. Data represent mean ± s.e.m. f, g Correlation of f miR-17-5p and g mir-18a-3p expression data from (d) and (e), respectively to BCL-2 protein levels measured by intracellular FACS. P values are determined by two-tailed Pearson’s correlation. h Intracellular FACS staining of BCL-2 protein levels in human APCKO organoids transduced with control, miR-17-5p, or miR-18a-3p anti-sense(AS) microRNA inhibitors (n = 3 independent experiments). Error bars indicate s.e.m. **p < 0.01, student’s t-test. i Correlation of miR-17 and BCL-2 expression in the TCGA COAD dataset.
