Fig. 1: SdeA partially localizes to the Golgi.
From: Serine-ubiquitination regulates Golgi morphology and the secretory pathway upon Legionella infection
A Schematic diagrams of full-length wild-type SdeA, SdeA catalytic mutant SdeA H277A or SdeA EE/AA, truncated SdeA1-972 and SdeA909-C. B Confocal images showing the colocalization of SdeA (green) with ER protein Calnexin (red). COS7 cells were transfected with plasmids encoding GFP-tagged wild type SdeA or truncated mutant. Cells were cultured for 24 h after transfection, then fixed, permeabilized, and stained with Calnexin antibody and visualized using confocal microscope. Scale bars, 10 μm. C Confocal images showing the colocalization of SdeA (green) with Golgi protein GM130 (red). Cells were cultured for 24 h after transfection, then fixed, permeabilized, and ultimately stained with GM130 antibody and visualized using confocal microscope. Scale bars, 10 μm.
