Fig. 2: Metastatic ascitic fluid is required for cell proliferation and generation of sMOCS.
From: Single cell-derived spheroids capture the self-renewing subpopulations of metastatic ovarian cancer
a Bright field images representative of the morphology of primary cultures derived from HGSOC ascites of different patients (x4 magnification for 2D monolayer culture of three different patients, scale bar 1000 μm). b Bright field image analysis (x4 magnification for patient 1, patient 2 and patient 3, scale bar 1000 mm) of a single cell from 3 different patients plated in STEM media only and the respective fluorescent signal for PKH67 + (green). c Bright field image analysis (x4 magnification for patient 1, scale bar 1000 μm; x20 magnification for patient 1, scale bar 200 μm) of a single cell from patient 1 cultured in STEM media supplemented with ascitic fluid and fluorescent signal for PKH67. d Time-lapse of a single well followed during cell division. Day 0: only a single cell is present in the well. Day 2: first mitotic division. Day 4: the small spheroid is formed. Day 5: the small spheroids divide in single cells. Day 6: all the cells derived from the original spheroid start to divide. Day 10: multiple monoclonal spheroids are grown.
