Fig. 4: single cell RNAseq reveals enrichment of CICs in sMOCS.
From: Single cell-derived spheroids capture the self-renewing subpopulations of metastatic ovarian cancer
a Number of cells for samples and patients analyzed after filtering and quality control. b UMAP of single cell transcriptomes from cells in (a), where each cell is represented by a point; each color tone identifies different conditions among the same patient, yellow tones: fresh ascites; turquoise tones: 2D culture; purple tones: sMOCS;. Top right: the magnification of the central area of the UMAP is enriched mainly for sMOCS cells but also for some fresh ascites and 2D cultured cells. c Diffusion map of single cell transcriptomes from cells in (a), where each cell is a point; yellow tones: fresh ascites; turquoise tones: 2D culture; purple tones: sMOCS. Bottom right: magnification of the region where all the samples from different patients and conditions converge, enriched in sMOCS cells. d–g Enrichment analysis of Cancer stem and EMT signature. Left: diffusion maps of all the conditions and patients with a color scale defined by the z score of the respective signature; right: frequency plot showing the variation in the distribution of cells as a function of the z score of the indicated signature. The higher the percentage of cells with high z score, the more enriched the sample is for the indicated signature.
