Fig. 5: Purified Bcl-xL, but not Bcl-xLK87D, suppresses IP3R1 single-channel opening. | Cell Death & Differentiation

Fig. 5: Purified Bcl-xL, but not Bcl-xLK87D, suppresses IP3R1 single-channel opening.

From: Bcl-xL acts as an inhibitor of IP3R channels, thereby antagonizing Ca2+-driven apoptosis

Fig. 5

Representative IP3R1 single-channel recordings from DT40-3KO cells ectopically expressing IP3R1. The channel opening was evoked by 1 µM IP3 (a–h), 100 nM IP3 (i to m) or 10 µM IP3 (n–p) at 200 nM Ca2+ and 5 mM ATP in the presence of PBS (a, i, n) or in the presence of 1 μM 6xHis-Bcl-2ΔTMD (b, j), 1 μM 6xHis-Bcl-xLΔTMD (c), 1 μM 6xHis-Bcl-xL (d, k, o), 0.3 μM 6xHis-Bcl-xL (e), 0.1 μM 6xHis-Bcl-xL (f), or 1 μM 6xHis-Bcl-xLK87D (g, l) purified proteins. Each vertical bar represents one single-channel current recording; C: closed; O: opened. Histogram depicting the Po ± SEM (N = 4 or 5) for the IP3R1 current recordings (h, m, p). Statistically significant differences were determined using a one-way ANOVA (*P < 0.05).

Back to article page