Fig. 1: High expression of the H2AZ isoforms correlates with GBM and GSC stemness. | Cell Death & Differentiation

Fig. 1: High expression of the H2AZ isoforms correlates with GBM and GSC stemness.

From: E2F and STAT3 provide transcriptional synergy for histone variant H2AZ activation to sustain glioblastoma chromatin accessibility and tumorigenicity

Fig. 1

Gene expression of selected H2A variants and canonical H2A in TCGA GBM (A) and GSCs (B). Only the top 4 canonical H2A genes (blue font) with the highest expression are shown. A *p < 1.0e−20 (Mann–Whitney test); B *p < 0.01 (two-tailed unpaired Student’s t test) denotes that the given variant H2A gene expression is significantly higher than expression of any of the shown canonical H2A genes. C Comparison of H2AZ mRNA levels in non-tumors vs. gliomas of different clinical grades in TCGA and REMBRANDT cohorts. Man–Whitney test, *p < 0.05, **p < 1.0e−04, ***p < 1.0e−12. D Correlative analysis of H2AZ levels with glioma patient survival in multiple glioma patient cohorts. OS overall survival. Wald test. E Correlative analysis of H2AZ levels with classical (CL), mesenchymal (MES) and proneural (PN) GBM subtypes in TCGA GBM. Man–Whitney test, **p < 0.01, ***p < 0.001. F Detection of H2AZ2 RNA transcripts in tumor cells from glioma patients of different clinical grades using the RNAscope assay. Scale bar, 10 µm. G RNAscope scores of H2AZ2 transcripts in different grades of glioma using glioma patient TMA (from F). H2AZ2 mRNA transcripts counts are binned into three score classes according to the RNAscope ® Assay Semi-Quantative Scoring Guideline. Differences in gene expression are estimated using Fisher-Freeman-Halton exact test. **p < 0.01, ***p < 0.001.

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