Fig. 1: Casp8^{ΔE385/ΔE385} mice are viable and develop a slight lymphopenia.

A Western blot of primary wild-type (WT) MDFs which were treated with TNF-α (40 ng/ml) +Cycloheximide (CHX) (40 μg/ml) (TC) for the indicated time. B Western blot of primary WT MDFs which were treated with TNF-α (20 ng/ml) +Smac mimetic (Smac) (1 μM) +zVAD (20 μM) (TSZ). C Western blot of RIPK1, RIPK3, MLKL, FADD, caspase-8, and GAPDH in the indicated organs of WT (1) and Casp8^{ΔE385/ΔE385} (2) mice. D Lymph nodes and spleens removed from 16-week old mice of indicated genotypes (scale bar, 1 cm). E Dot plot of weight of lymph nodes (parts showed in Fig. 1D) and spleens of 12- to 16-week old WT, Casp8^{ΔE385/ΔE385} mice. Bars, mean ± SD. P values above the asterisk (unpaired, two-tailed t test) ****p < 0.0001, compared to the WT mice. F Different cell subsets from spleen, lymph nodes (parts showed in Fig. 1D) and bone marrow of 12- to 16-week old WT and Casp8^{ΔE385/ΔE385} mice were analyzed by flow cytometry using the following markers: B cells (B220⁺ or CD19⁺), T cells (CD3⁺), CD4⁺ T cells (CD3⁺CD4⁺CD8⁻), CD8⁺ T cells (CD3⁺CD8⁺CD4⁻), Granulocytes and Macrophages (CD11b⁺), mature B cells in spleen (B220⁺IgM⁺ or B220⁺CD19⁺), immature and mature B cells in bone marrow (B220⁺ IgM⁺ or B220^hi CD19^hi), progenitor B cells (pro-B) and precursor B cells (pre-B) in bone marrow (B220⁺ IgM⁻ or B220^low CD19^low). Bars, mean ± SD. P values above the asterisk (unpaired, two-tailed t test) *p < 0.05, **p < 0.05, ****p < 0.0001.