Fig. 6: NOTCH inhibition restores multiciliation in CP tumors. | Cell Death & Differentiation

Fig. 6: NOTCH inhibition restores multiciliation in CP tumors.

From: Disruption of GMNC-MCIDAS multiciliogenesis program is critical in choroid plexus carcinoma development

Fig. 6

Representative images of immunofluorescent staining for ARL13B (A, yellow; B, red) are shown in tumor cells at day E17.5 (A) and tumor cells isolated at day P7 (B) from Lcre;NICD1 (A, B) and Lcre;Ptchcko;NICD1 (A) animals treated with vehicle or IMR-1 from day E10.5 to day E16.5. Boxed region of ciliated cells is magnified in lower panel (A). DAPI staining (A, cyan; B, blue) labels nuclei. Scale bars, 5 µm (A), 10 µm (B). Results were obtained from at least three independent experiments. C Quantification total tumor cell numbers isolated at day P7 is shown in Lcre;NICD1 animals treated as described in A and B (n = 5 animals per treatment; mean ± s.e.m., two-tailed unpaired t-test, ***P < 0.001). D The expression of Ki-67 (red) in NICD1+/GFP+ tumor cells at day E17.5 is shown in Lcre;NICD1 animals treated with vehicle or IMR-1 from day E10.5 to day E16.5. DAPI staining (blue) labels nuclei. Scale bar, 20 µm. Quantification of Ki-67 expression in tumor cells is shown (right panel: n = 9 animals for vehicle, n = 8 animals for IMR-1; mean ± s.e.m., two-tailed unpaired t-test, **P < 0.01). Data are representative of three independent experiments.

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