Fig. 1: Loss of CUL4B at the crypt base disturbs intestinal cryp-villi structure.
From: Cullin 4b-RING ubiquitin ligase targets IRGM1 to regulate Wnt signaling and intestinal homeostasis
a The representative images of CUL4B staining on small intestine (SI) tissue. Green, CUL4B, Blue, DAPI. The scale bar is 50 μm (Left) and 20 μm (Right). b Western blots analysis of CUL4B, the stem cell marker (Lgr5), and the villi marker (CK20) in extracts from crypt and villus of the SI. c Western blots analysis of CUL4B, the nuclear marker (Histone protein, H3), and the cytoplasm marker (α-Tubulin) in cytoplasmic and nuclear extracts from crypts of the SI. d The percentage of CUL4B positive staining by statistical quantification of cell position along the crypt-villus from the bottom. e The representative image of co-staining of Lgr5 and CUL4B in the crypts of Lgr5-EGFP-ires-CreERT2 mouse. The white arrowhead, co-expression of Lgr5 and CUL4B. The red arrowhead, exclusive expression of CUL4B. The green arrowhead, exclusive expression of Lgr5. The scale bar is 10 μm. f The representative image of co-staining of Lyz and CUL4B in the crypts. The scale bar is 50 μm. g The measurement of intestine weight of duodenum, jejunum plus ileum, colon of KOIEC (N = 4), and WT mice (N = 4). Error bars represent standard errors. **P < 0.01, *P < 0.05. h The representative images of H&E staining of sections of SI from WT and KOIEC mice. The scale bar is 100 μm(left), 25 μm(right). i, j The Measurement of villus length (i) and crypt length (j) of KOIEC, Tg and WT mice (N = 3). Error bars represent standard errors. ****P < 0.0001.
