Fig. 3: Lgr5+ stem cells and secretory lineage differentiation are reduced after CUL4B ablation.
From: Cullin 4b-RING ubiquitin ligase targets IRGM1 to regulate Wnt signaling and intestinal homeostasis
a The mRNA expression level of Lgr5 and Alpi isolated from KOIEC intestine crypts relative to WT littermates, KOIEC organoids relative to WT and KOCAG organoids relative to WT. *P < 0.05, ****P < 0.0001. b The representative images of GFP staining in SI of KOLgr5 mice and WT littermates. (N = 3). The scale bar is 50 μm. ****P < 0.0001. c The mRNA expression level of stem cell markers in KOIEC crypts relative to WT littermates. *P < 0.05, **P < 0.01, ****P < 0.0001. d The representative images of Lyz staining in SI of KOIEC mice and WT littermates (N = 3). The scale bar is 50 μm. ****P < 0.0001. e The mRNA expression level of Lyz in KOIEC crypts relative to WT littermates. *P < 0.05. f The representative images and quantification of Mucin2 staining in SI of KOIEC and WT mice (N = 3). The scale bar is 50 μm. ****P < 0.0001. g The representative images and quantification of ChgA staining in SI of KOIEC mice and WT littermates (N = 3). The scale bar is 50 μm. **P < 0.01. h The mRNA level of enterocytes markers Alpi and Apoa4 in KOIEC crypts relative to WT littermates. *P < 0.05, **P < 0.01. i The mRNA level of Krt20 in KOIEC crypts relative to WT littermates. *P < 0.05. Error bars represent standard errors in the figure.
