Fig. 2: ACLC is inhibited by ATP and gated by F1.
From: Mitochondrial ATP synthase c-subunit leak channel triggers cell death upon loss of its F1 subcomplex
A, B Representative lipid bilayer recordings of human c-ring before and after the addition of ANT inhibitor bongkrekic acid (BA, 5 µM, −90 mV holding voltage) and ATP (1 mM, −100 mV holding voltage). C refers to closed state of the channel activity, O refers to open state of channel activity. C Representative trace of an excised proteoliposome patch recording of c-ring before and after the addition of F1 (5 µg, final concentration, −100 mV holding voltage). D Representative lipid bilayer recordings of c-ring before and after the addition of F1 (5 µg, +120 mV holding voltage) or E α3β3 complex (5 µg, −80 mV holding voltage). F Group data for peak conductances of c-subunit lipid bilayer recordings in response to ATP (n = 5) or BA (n = 10). ****P < 0.0001 (paired t-test), in which each channel recording was compared before and after reagent addition. For simplicity of presentation, the control data are grouped in the same bar. G Group data for peak conductances of c-subunit lipid bilayer recordings in response to purified F1 (n = 6), boiled F1 (n = 4) and α3β3 complex (10); ****P < 0.0001, paired t-test; as in F the control data from each paired study are grouped into one bar for simplicity of presentation. H Representative excised patch proteoliposome recordings of the c-subunit channel during continuous ramp voltage clamp from −100 mV to +100 mV before and after F1 addition.
