Fig. 2: SIRT7 can be O-GlcNAcylated by OGT. | Cell Death & Differentiation

Fig. 2: SIRT7 can be O-GlcNAcylated by OGT.

From: O-GlcNAcylation and stablization of SIRT7 promote pancreatic cancer progression by blocking the SIRT7-REGγ interaction

Fig. 2

A Affinity purification of SIRT7 interacting proteins from HEK293T cell stably expressing Flag-SIRT7. SDS-PAGE and Coomassie Brilliant Blue staining shows proteins co-eluted with SIRT7. The black arrow points to SIRT7, and the red arrow points to the differential band of interest. The differential band was cut for analysis by LC-MS/MS, and a series of peptides that contained OGT were detected at 110kD. B Co-IP assays were performed in HEK293T cells transfected with the HA-OGT, HA-OGT-908A or Flag-SIRT7 plasmids. One representative experiment of n = 3 independent experiments is shown. C Co-IP assays of OGT and SIRT7 were examined in PANC-1 and MiaPaCa-2 cells. One representative experiment of n = 3 independent experiments is shown. D Representative IHC staining of OGT, SIRT7 and Duolink® PLA brightfield signals (red arrows) in one PDAC patient (consecutive sections). Scale bar as shown. One representative experiment of n = 3 independent experiments is shown. E Schematic drawing of full-length SIRT7 and truncations. F Schematic drawing of full-length OGT and truncations. G Co-IP assays were performed in HEK293T cells transfected with the HA-OGT and Flag-SIRT7 truncations. One representative experiment of n = 3 independent experiments is shown. H, I Co-IP assays were performed in HEK293T cells transfected with the Flag-SIRT7 and HA-OGT truncations. One representative experiment of n = 3 independent experiments is shown.

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