Fig. 5: Loss of SIRT7 O-GlcNAcylation inhibits expression of target genes via hyperacetylation of H3K18. | Cell Death & Differentiation

Fig. 5: Loss of SIRT7 O-GlcNAcylation inhibits expression of target genes via hyperacetylation of H3K18.

From: O-GlcNAcylation and stablization of SIRT7 promote pancreatic cancer progression by blocking the SIRT7-REGγ interaction

Fig. 5

A Western analysis of multiple histone acetylation sites of H3, H4 in PANC-1 and MiaPaCa-2 cells treated with SIRT7 knockdown. One representative experiment of n = 3 independent experiments is shown. B Western analysis of multiple histone acetylation sites of H3, H4 in PANC-1 and MiaPaCa-2cells transfected with NC, HA-OGT or HA-OGT-908A. One representative experiment of n = 3 independent experiments is shown. C Western analysis of multiple histone acetylation sites of H3, H4 in PANC-1 and MiaPaCa-2cells transfected with HA-OGT or/and siSIRT7. One representative experiment of n = 3 independent experiments is shown. D The expression of SIRT7 target genes in the PANC-1 cells treated with SIRT7 knockdown, as determined by qPCR. One representative experiment of n = 3 independent experiments is shown. E The expression of SIRT7 target genes in the PANC-1 cells transfected with NC, HA-OGT or HA-OGT-908A, as determined by qPCR. One representative experiment of three independent experiments is shown. F ChIP-qPCR results showing H3K18Ac occupancy at the promoters of SIRT7 target genes in control or OGT knockdown PANC-1 cells. One representative experiment of n = 3 independent experiments is shown. G ChIP-qPCR results showing SIRT7 occupancy at the promoters of SIRT7 target genes in control or OGT knockdown PANC-1 cells. One representative experiment of n = 3 independent experiments is shown. The data are shown as the means ± SD. P values were calculated by two-tailed t tests (*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; NS, no significance).

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