Fig. 5: Expression of either NDI1 or SIRT3 impairs tumorigenicity of MPNST cells. | Cell Death & Differentiation

Fig. 5: Expression of either NDI1 or SIRT3 impairs tumorigenicity of MPNST cells.

From: Tumor growth of neurofibromin-deficient cells is driven by decreased respiration and hampered by NAD+ and SIRT3

Fig. 5

Representative OCR traces and quantification of basal OCR of sMPNST (A) and cisMPNST (B) cells. The ATP synthase inhibitor oligomycin (0.8 µM), the proton uncoupler carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP, 1 µM) and the respiratory complex I and III inhibitors rotenone (0.5 µM) and antimycin A (1 µM), respectively, were added where indicated. C Effect of NDI1 expression on soft agar colony formation of sMPNST (left) and cisMPNST (right) cells. SIRT3 overexpression after transfection with pFUGW-SIRT3 (D) decreases Matrigel colony formation of MPNST cells (E). In D, actin was used as a loading control. All along the Figure, the MPNST cell models sMPNST and cisMPNST were used. NDI: cells expressing the pWPI-NDI1 construct; EV: cells expressing the pWPI empty vector; SIRT3: cells expressing pFUGW-SIRT3; GFP: cells expressing pFUGW-GFP. Data are reported as mean ± SD values (n ≥ 3); ***p < 0.001; **p < 0.01 and *p < 0.05 with a Student’s t test analysis.

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