Fig. 5: Combined BCL-xL and MCL-1 inhibition causes apoptosis in human GBM ex vivo.
From: Increased apoptotic sensitivity of glioblastoma enables therapeutic targeting by BH3-mimetics
A–C Representative images of H&E, Ki67 and cleaved Caspase 3 IHC of three patients diagnosed with IDHwt GBM (case 1–3; ctrl = DMSO, dual treatment = A-1331852 1 μM + S63845 1 μM) (D–F) Quantification of cellularity, percentage Ki67 and SOX2 positive cells/total cell count in all three cases treated with the drugs described in (A–C) for 72 h. Error bars represent mean ± SEM (***p = 0.0009) Welch’s test. G Representative images of IF staining of GFAP, NESTIN (both red) and cleaved Caspase 3 (green) in the subventricular zone (SVZ) of mouse brain slices, cultured and treated with DMSO or a combination of ABT-263 5 μM and S63845 2 μM for 24 h, counterstaining with DAPI (blue). H Quantification of cleaved Caspase 3 positive cells normalised to nuclear count in single mice. Single dots represent analysed images. Error bars represent median (mouse 1: ns p = 0.9198, p = 0.3086; mouse 2: ns p = 0.149, p = 0.2974; mouse 3: ns p = 0.0593, **p = 0.0044) Welch’s test. Scale bars = 50 µm.
