Abstract
Hematopoiesis occurs within a unique bone marrow (BM) microenvironment, which consists of various niche cells, cytokines, growth factors, and extracellular matrix components. These multiple components directly or indirectly regulate the maintenance and differentiation of hematopoietic stem cells (HSCs). Here we report that BAP1 in BM mesenchymal stromal cells (MSCs) is critical for the maintenance of HSCs and B lymphopoiesis. Mice lacking BAP1 in MSCs show aberrant differentiation of hematopoietic stem and progenitor cells, impaired B lymphoid differentiation, and expansion of myeloid lineages. Mechanistically, BAP1 loss in distinct endosteal MSCs, expressing PRX1 but not LEPR, leads to aberrant expression of genes affiliated with BM niche functions. BAP1 deficiency leads to a reduced expression of pro-hematopoietic factors such as Scf caused by increased H2AK119-ub1 and H3K27-me3 levels on the promoter region of these genes. On the other hand, the expression of myelopoiesis stimulating factors including Csf3 was increased by enriched H3K4-me3 and H3K27-ac levels on their promoter, causing myeloid skewing. Notably, loss of BAP1 substantially blocks B lymphopoiesis and skews the differentiation of hematopoietic precursors toward myeloid lineages in vitro, which is reversed by G-CSF neutralization. Thus, our study uncovers a key role for BAP1 expressed in endosteal MSCs in controlling normal hematopoiesis in mice by modulating expression of various niche factors governing lymphopoiesis and myelopoiesis via histone modifications.
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Data availability
The raw RNA-seq and ChIP-seq data generated in this study have been deposited in NCBI Gene Expression Omnibus database (accession numbers of GSE198823 for RNA-seq data and GSE198649 for ChIP-seq data).
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Acknowledgements
We thank members of our laboratories for providing reagents and helpful advice. We thank Hyeong-In Ham for assistance with the manuscript preparation.
Funding
This work was supported in part by the National Research Foundation of Korea (NRF-2021R1A2B5B03002202), Korea Mouse Phenotyping Project (NRF-2014M3A9D5A01073789) of the Ministry of Science, Information and Communication Technology, and Future Planning through the National Research Foundation (to RHS); and the National Research Foundation of Korea (NRF-2018R1A6A1A03025810), the Research Fund of UNIST (1.220023.01) (to MK).
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JJ designed and performed experiments. IJ, JHK., SJ, HM, BK, JN performed the experiments. DYH and DH analyzed the ChIP-seq data. SJU provided critical materials. RHS and MK conceived and supervised the study. JJ, MK, and RHS wrote and edited the manuscript. All authors reviewed and approved the manuscript.
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All animal procedures were approved by and conducted according to the Institutional Animal Care and Use Committee (IACUC) at Seoul National University.
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Jeong, J., Jung, I., Kim, JH. et al. BAP1 shapes the bone marrow niche for lymphopoiesis by fine-tuning epigenetic profiles in endosteal mesenchymal stromal cells. Cell Death Differ 29, 2151–2162 (2022). https://doi.org/10.1038/s41418-022-01006-y
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DOI: https://doi.org/10.1038/s41418-022-01006-y
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