Fig. 7: EHF and CDX1 have overlapping expression profiles across intestinal epithelial cell subtypes and are co-ordinately methylated in CRCs. | Cell Death & Differentiation

Fig. 7: EHF and CDX1 have overlapping expression profiles across intestinal epithelial cell subtypes and are co-ordinately methylated in CRCs.

From: Epithelial de-differentiation triggered by co-ordinate epigenetic inactivation of the EHF and CDX1 transcription factors drives colorectal cancer progression

Fig. 7

A t-SNE plots of EHF and CDX1 mRNA expression in different intestinal cell types determined by interrogation of the Haber et al. dataset [20] using the single cell expression atlas. B, C Pearson’s correlation of B EHF (cg05503887, cg18414381 and cg18560551) and C CDX1 (cg11524248, cg24216701, cg25132276, cg26531174 and cg11117637) mean promoter methylation and mRNA expression in primary colorectal tumours (n = 376). Data obtained from the TCGA portal. D, E Methylation profiling of the (D) EHF gene body and E CDX1 promoter in four moderately-differentiated (MD) and four poorly-differentiated (PD) CRC cell lines determined using human methylation arrays. Value shown are the mean methylation value (Beta values) from a single methylation profiling array of each cell line. F Pearson’s correlation of EHF and CDX1 methylation in primary colorectal tumours from the TCGA cohort (n = 376). G, H Effect of 5-aza-2’-deoxycytidine (Decitabine, DAC) treatment (1 µM) on EHF and CDX1, and differentiation marker mRNA expression in G HCT116 and H RKO cells. q-RT-PCR data shown are mean ± SEM from a representative experiment performed in triplicate. *p < 0.05; **p < 0.01, Student’s t test.

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