Fig. 2: TRAP1 interaction with OSCP and CyPD.
From: The mitochondrial chaperone TRAP1 regulates F-ATP synthase channel formation
A Gel filtration chromatography analysis of TRAP1 distribution in mitochondria of human U87 glioblastoma cells. OSCP and β subunits were used to assess the presence of F-ATP synthase. B BN-PAGE performed on isolated mitochondria (left) showing F-ATP synthase monomers and dimers (M and D, respectively). M and D bands were cut, run on SDS-PAGE and probed for the presence of F-ATP synthase subunits γ and OSCP, TRAP1 and CyPD. C, D Tripartite split GFP experiment on sMPNST cells co-transfected with GFP1–9 fused to a mitochondrial import sequence, together with GFP10-TRAP1 and GFP11-OSCP (C), or with GFP10-TRAP1 and GFP11-CyPD (D). Green dots indicate mitochondrial interaction between TRAP1 and OSCP (C) or TRAP1 and CyPD (D). MitoTracker (red dots) was used to stain mitochondria; superimposition of MitoTracker and GFP signal is shown as yellow dots and indicates that protein interaction occurs in mitochondria.
