Fig. 6: Opposite effects and competition of TRAP1 and CyPD on F-ATP synthase channel activity.
From: The mitochondrial chaperone TRAP1 regulates F-ATP synthase channel formation
A–D High conductance, flickering channel activity detected after direct incorporation of 16 µg F-ATP synthase in the planar lipid bilayer and addition of 3 mM Ca2+ (A; Vcis = −60 mV). The effects on channel activity of subsequent additions of CyPD (B; molar ratio with F-ATP synthase of 2:1) and TRAP1 (C: molar ratio with F-ATP synthase of 1:1; D: molar ratio with F-ATP synthase of 2:1) are reported. E–H High conductance, flickering channel activity detected in the presence of 3 mM Ca2+ (E; Vcis = −60 mV). The effects on channel activity of subsequent additions of TRAP1 (F; molar ratio with F-ATP synthase of 2:1) and CyPD (G: molar ratio with F-ATP synthase of 2:1; H: molar ratio with F-ATP synthase of 5:1) are reported. In all figure panels, the prevalent conductance levels are marked with dotted lines and the conductance values are indicated; the closed state is denoted with C. Either TRAP1 or CyPD were added at increasing concentrations after Ca2+-induced channel activity was detected.
