Fig. 1: C8-endo mice succumb to small intestinal hemorrhage.

a The proportion of mice that became moribund and required euthanasia. Gray boxes indicate days of tamoxifen administration. Data are combined from five independent experiments (n = 23–27 mice). ****P < 0.0001 via Log-rank Mantel–cox test. b Representative images of the gastrointestinal tract from a moribund C8-endo mouse and matched C8-flox and wild-type mice. c Representative images of the luminal face of the ileum from C8-endo mice 12 or 16 days after commencing tamoxifen treatment. Open arrow indicates a nascent hemorrhagic lesion. Asterisks indicate larger more developed hemorrhagic lesions. d Relative changes to the body weight of mice. Mean ± s.d. from n = 5 mice per genotype are shown. Gray boxes indicate days of tamoxifen administration. e Representative H&E-stained sections of the small intestines of control (C8-flox) mice and C8-endo mice 10, 12 and 16 days post-tamoxifen. Yellow arrowheads indicate compromised blood vessels. Asterisk indicates hemorrhagic transformation. Arrows indicate empty crypts. Black arrowhead indicates a Goblet cell. f Immunofluorescence images of sections from C8-endo-ROSA mice five days after the first dose of tamoxifen. Right panels are enlarged micrographs of the inset white boxes in the left-most panels. Scale bars in left panels indicate 100μM. Scale bars in right panels indicate 30 μM. Arrowheads indicate GFP-negative endothelial cells. Micrographs are representative of at least six specimens per group. g Quantification of the percentage of EGFP-CD31-positive cells over the total number of CD31-positive cells across the small and large intestine of C8-endo mice. Data are the mean ± s.e.m. of >100 cells/group/mouse. Symbols represent individual C8-endo mice. ns via one-way ANOVA with Geisser-Greenhouse correction.