Fig. 3: p53 phosphorylation mediates SIRT4-induced autophagy in PDAC.
From: Sirtuin 4 activates autophagy and inhibits tumorigenesis by upregulating the p53 signaling pathway
A KEGG analysis of the differentially expressed genes in Capan-2 cell with or without SIRT4 overexpression. B The intersections of pathways from metabolomics and RNA-seq with SIRT4-overexpressed Capan-2 cells, and proteomics in KSC mice. Western blotting analysis was used to detect the phosphorylation level of p53 protein and the expressions of downstream target genes in mouse primary cells (C), CCE-HPE-2 (D), and HPDE6-C7 cells (E). Western blotting analysis was used to detect the phosphorylation level of p53 protein and the expressions of downstream target genes in Capan-2 (F) and Hs776T cells (G). Detection of glutamine uptake (H), NH4+ production (I), lactate production (J), and glucose uptake (K) in primary pancreatic cells of WT mice and SIRT4−/− mice. In the absence or presence of glutamine metabolic inhibitors, western blotting analysis was used to detect the phosphorylation level of p53 protein and the expressions of downstream target genes in mouse primary cells (L) and CCE-HPE-2 cells (M). GraphPad software was used for independence Sample t-test, (*P < 0.05; **P < 0.01; ***P < 0.001).
