Fig. 6: AMPKα plays a key role in the suppression of pancreatic development by SIRT4.

A, B Representative images and its quantification of sphere formation assay to detect the tumorigenic ability of CCC-HPE-2 and HPDE6-C7 cells transfected with scramble or sh-SIRT4 vectors, respectively, in the absence or presence of platycodin D, an AMPK activator. C, D Representative images and its quantification of sphere formation assay to detect the tumorigenic ability of Capan-2 and Hs766T cells bearing control or SIRT4 vectors, respectively, in response to AMPK depletion. Representative ventral view images of bioluminescence from nude mice subcutaneously injected with CCC-HPE-2 (E) and Capan-2 cells (F). G SEM was applied to detect autophagy in tumor tissues of nude mouse xenograft tumor models. H Western blotting analysis was used to detect the expressions of ARGs in tumor tissues of nude mouse xenograft tumor models. GraphPad software was used for the independence sample t-test (*P < 0.05; **P < 0.01).