Fig. 2: Caspase-8 is activated after HSV-1 infection and required for HSV-1-induced caspase-3 activation and apoptosis in U937 cells. | Cell Death & Differentiation

Fig. 2: Caspase-8 is activated after HSV-1 infection and required for HSV-1-induced caspase-3 activation and apoptosis in U937 cells.

From: Caspase-8 is required for HSV-1-induced apoptosis and promotes effective viral particle release via autophagy inhibition

Fig. 2

A Caspase-8 (IETDase) activity assay of total extracts of wt U937 cells infected with 50 m.o.i. of HSV-1 for 0 to 36 h. B Caspase-3 (DEVDase) activity assay on total extracts of wt and caspase-8−/− (Casp-8−/−) U937 cells infected with HSV-1 for 0 (NT), 10 and 24 h. C Anti-caspase-8, -caspase-3 and -gD western blot analyses of total extracts of wt and caspase-8−/− U937 cells infected with HSV-1 for 0, 10 and 24 h showing the inactive p55 pro-form and the active p43/p41 and p18/p10 caspase-8 fragments, the active p20/p17 caspase-3 (Cl Casp-3) fragments and the expression of the viral gD protein. β-Actin served as a loading control. D Percentage of FITC-Annexin-V/7-AAD-negative (surviving) wt and caspase-8−/− (Casp-8−/−) U937 cells infected with HSV-1 for 0 (NT), 10 and 24 h. Data in A, B and D represent the means of 3–7 independent experiments ± SD. Statistical evaluation by one-way ANOVA: **p < 0.01, ***p < 0.001.

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