Fig. 3: Mouse p53 mRNA structure was not altered during ER stress.

a Nucleotide sequence alignment of human (+1 to +535 nts) and mouse (+1 to +517 nts) p53 mRNA CDS, positions of 1st and 2nd AUGs are marked with lines. Circular plots of the mouse p53 mRNA base-pairing potentials based on the SHAPE reactivity under normal (DMSO) (b) and ER stress (Thap) conditions (c). Arc plots show the probability of base pairings observed in circular plots (a and b) under normal (d) and ER stress conditions (e). Secondary structures of the mouse p53 mRNA coding sequences from +1 to +517 nts mapped using the SuperFold algorithm based on the SHAPE values under normal (f) and ER stress conditions (g), SHAPE modified nucleotides are indicated in orange and red.